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Polyclonal Secondary antibodies (whole molecules)

Detection step for murine primary antibodies IgG and/or IgM
anti MOUSE IgG+IgM “human ads.” FITC
Polyclonal Liquid 0.5 mL

Ref 50-010

document(s)
anti MOUSE IgG+IgM “human ads.” FITC
Polyclonal Liquid 1 mL

Ref 50-011

document(s)
anti MOUSE IgG+IgM “human ads.” FITC
Polyclonal Liquid 2 mL

Ref 50-012

document(s)
anti MOUSE IgG+IgM “human ads.” PEROXIDASE
Polyclonal Liquid 0.5 mL

Ref 50-020

document(s)
anti MOUSE IgG+IgM “human ads.” PEROXIDASE
Polyclonal Liquid 1 mL

Ref 50-021

document(s)
anti MOUSE IgG+IgM “human ads.” PEROXIDASE
Polyclonal Liquid 2 mL

Ref 50-022

document(s)
These Goat polyclonal antibodies are affinity purified and adsorbed against human proteins in order to avoid cross reactivity on human origin tissue. Depending of their labelling, they are suitable for use in: immunofluorescence, immunoperoxidase, western blot, flow cytometry, ELISA... In case blood cells are present in the studied specimens, preferably use F(ab’)2 fragment antibodies in order to avoid non specific binding due to Fc receptors. Recommended working dilutions: FITC-Microscopy: 1/50-1/250, HRP-Microscopy: 1/50-1/250, HRP-ELISA: 1/1000-1/5000, HRP-Blotting: 1/500-1/3000.
For research use only.

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Polyclonal Secondary antibodies F(ab’)2

Detection step for murine primary antibodies IgG and/or IgM
F(ab’)2 anti MOUSE IgG+IgM “human ads.” FITC
Polyclonal Liquid 0.5 mL

Ref 51-010

document(s)
F(ab’)2 anti MOUSE IgG+IgM “human ads.” FITC
Polyclonal Liquid 1 mL

Ref 51-011

document(s)
F(ab’)2 anti MOUSE IgG+IgM “human ads.” FITC
Polyclonal Liquid 2 mL

Ref 51-012

document(s)
F(ab’)2 anti MOUSE IgG+IgM “human ads.” PEROXIDASE
Polyclonal Liquid 0.5 mL

Ref 51-020

document(s)
F(ab’)2 anti MOUSE IgG+IgM “human ads.” PEROXIDASE
Polyclonal Liquid 1 mL

Ref 51-021

document(s)
F(ab’)2 anti MOUSE IgG+IgM “human ads.” PEROXIDASE
Polyclonal Liquid 2 mL

Ref 51-022

document(s)
These Goat polyclonal antibodies are affinity purified and adsorbed against human proteins in order to avoid cross reactivity on human origin tissue. Depending of their labelling, they are suitable for use in: immunofluorescence, immunoperoxidase, flow cytometry... Recommended working dilutions: FITC-Microscopy: 1/50-1/250, HRP-Microscopy: 1/50-1/250.
For research use only.

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PBS pH: 7.2

Washing and dilution buffer
PBS pH: 7.2 without Ca2+ and Mg2+
Powder - 2 x 1 Liter

Ref 33-010

document(s)
PBS pH: 7.2 without Ca2+ and Mg2+
Powder - 4 x 1 Liter

Ref 33-011

document(s)
This buffer is suitable for dilution and/or washing steps of almost all antibodies listed in this catalog (refer to products package insert). The fluorescence with Fluorescein Isothiocyanate labelled antibodies is maximized when the pH is slightly alkaline (pH 7.2). Reconstitution: Each vial contains sufficient powder for 1 liter of ready to use PBS. Use only distilled water for reconstitution. Composition For 1 liter: NaCl:7.650 g / Na2HPO4:724 g / KH2PO4: 0.210 g.
For research use only.

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Evan’s blue

Immunofluorescence counterstaining
Evans blue 1%
Liquid - 2 mL

Ref 33-030

document(s)
Provides red contrasting of cells in immunofluorescence. Reconstitution: Evans blue 1% is used in the range of 1/50 to 1/500 diluted in PBS (1/5 000-1/50 000 final).
For research use only.

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Immunofluorescence slides

Samples preparation for microscopy
IF slides - Teflon coated - 6 mm wells
10 wells - 100 slides

Ref 31-010

Teflon coated slides 10 wells 6 mm diameter. These slides are suitable for use with acetone or methanol and for deep-freeze.


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Mounting medium for immunofluorescence

Mounting microscope preparations
Mounting medium for immunofluorescence
Liquid 15 mL dropper bottle

Ref 33-041

document(s)
Buffered glycerol solution recommanded for the use of Argene monoclonal antibodies in immunofluorescence for mounting coverslips over microscope preparations
For research use only.

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Mounting medium for immunofluorescence FLUOKEEP™

Stick microscope coverslips and conserve immunofluorescence
Fluokeep (mounting medium for IF)
Liquid - 15 mL

Ref 33-040

document(s)
Gelatinous solution of polyvinylic alcohol, glycerol, and Tris buffer designed for fixing coverslips over microscopic preparation, particularly for Immunofluorescence. With FLUOKEEP™ the preparation can be stored without loss of fluorescence for a few days stored at +2/+8°C.
For research use only.

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Kova glasstic slides for CMV antigenemia

Count for leukocytes
“Kova glasstic slides” for antigenemia
10 wells - 100 slides

Ref 31-030

Single use 10 wells plastic slides to count the leukocytes before the cytocentrifugation in the CMV pp65 antigenemia technic.


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Antibodies coating buffer pH 9.6

Antibody & protein coating on plastic
Coating buffer pH 9.6 for antibodies
Tablets - 4 x 100 mL

Ref 33-070

document(s)
This buffer is suitable for the coating of antibodies on plastic (microplates, beads). Each tablet is sufficient for 100 mL of ready to use buffer. Reconstitution: Each tablet is dissolved in 100 mL of distilled water.
For research use only.

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AEC Aminoethyl Carbazole / substrate kit

Peroxidase substrate
AEC complete kit
Liquid - 3 x 12 mL

Ref 33-050

document(s)
This kit allows preparation of AEC substrate simply by mixing 1 drop of each of the 3 reagents in 1 mL of distilled water. This substrate will produce a red colored deposit upon reaction with peroxidase. Kit content: A. 1 dropper bottle (12 mL) of buffer concentrate (20x), B. 1 dropper bottle (12 mL) of chromogen concentrate (20x), C. 1 dropper bottle (12 mL) of concentrate hydrogen peroxide (20x). Preparation of substrate / chromogen mixture: To 1 mL of distilled water add 1 drop of reagent A (mix well), 1 drop of reagent B, 1 drop of reagent C (mix again). The substrate/chromogen mixture is ready to use. This solution should be kept away from light and used within 30 min. Recommended guidelines for use: Incubate the substrate/chromogen mixture with the specimen for 3-5 mn. If desired incubation times up to 30 mn can be done with satisfactory results. Color development may be monitored under a microscope. After the color is adequately developed, the specimen may be counterstained and mounted with an aqueous or permanent mounting solution.
For research use only.

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Updated/Mise à jour 2010-06-03 08:24:03