The optimized blend of murine clones (CINApool) is specific for the internal matrix structural phosphoprotein 65-68 kDa ppUL83 (protein kinase, pp65 or pk65), which appears in the nuclei of infected cells within one hour post infection, and represents uptake from the viral innoculum. This blend labels two different epitopes expressed in the nuclei of infected peripheral blood polymorphonuclear leukocytes and monocytes, following blood born dissemination (It can also be used on cellular cerebro-spinal fluid). The HCMV isolation from leukocytes of peripheral blood and/or ppUL83 Antigenaemia are evidences for active systemic infection. The ppUL83 Antigenemia appears earlier and is positive for a longer period than viremia as demonstrated by co-culture. It is also a clearly shorter procedure (results within 2 hours). It enables the diagnosis and follow up of HCMV disseminated infections in graft recipients and AIDS patients. Immunofluorescence is the only detection method recommended, although others can be used. However different studies show that any technique used to block endogenous enzymatic activity (peroxidase or alkaline phosphatase), also destroys a significant level of the ppUL83 antigen. No cross-reactivity with other Herpesviridae. This blend has multiple advantages: 1/ This antibody pool labels different epitopes expressed in the nuclei of infected peripheral blood polymorphonuclear leukocytes and monocytes, and thus ensures avoiding false negatives that can theoretically be caused by mutation of one of the epitope recognized since a double mutation in two specific parts of the antigen is at very low risk. 2/ The reading is improved particularly if the sample has not reached the laboratory under optimum conditions 3/ This anti ppUL83 pool is also of interest since it stains a higher number of cells in positive samples when compared to a monoclonal alone. Important: Secondary antibodies other than the one recommended can give cytoplasmic background. SEE ALSO CONTROL SLIDES

This murine monoclonal antibody is specific for the internal matrix structural phosphoprotein 65-68 kDa ppUL83 (pp65 or pk65), which appears in the nuclei of infected cells within one hour post infection, and represents uptake from the viral innoculum. This antibody labels one of the epitopes expressed in the nuclei of infected peripheral blood polymorphonuclear leukocytes and monocytes, following blood born dissemination (It can also be used on cellular cerebro-spinal fluid).The HCMV isolation from leukocytes of peripheral blood and/or ppUL83 Antigenemia are evidences for active systemic infection. The ppUL83 Antigenemia appears earlier and is positive for a longer period than viremia as demonstrated by co-culture. It is also a clearly shorter procedure (results within 2 hours). It enables the diagnosis and follow up of HCMV disseminated infections in Graft recipients and AIDS patients. Immunofluorescence is recommanded as different studies show that any technique used to block endogenous enzymatic activity (peroxidase or alkaline phosphatase) also destroys a significant level of the ppUL83 antigen. No cross-reactivity with other Herpesviridae. Important: Secondary antibodies other than the one recommended can give cytoplasmic background. SEE ALSO CONTROL SLIDES.

This pool of murine monoclonal antibodies improves the sensitivity of antigen detection in culture at 48 hours post infection and of direct immunofluorescence slide test in BAL. The first antibody recognizes an Immediate Early Antigen which can be detected 2 h post infection, the second antibody recognizes an Early Antigen which can be detected 12 hours post infection. Both antibodies give nuclear staining. No cross-reactivity with other Herpesviridae. Suitable for use in cultures from blood (Buffy coat), BAL, CSF, urine, amniotic fluid and in immunohistochemistry on lung, liver, kidney, colon, brain, nerve... SEE ALSO CONTROL SLIDES.

This murine monoclonal antibody recognizes the Immediate Early non structural proteins 52, 72 and 86 kDa . The common epitope labelled is encoded by exon 2 of the Major Immediate Early gene. This antigen appears 2 hours after cell infection, reaches the intensity peak at 48 hours and persists during the entire HCMV infection cycle. It gives a specific nuclear fluorescence. No cross-reactivity with HSV-1, HSV-2, VZV, EBV and ADV. Suitable for use in cultures from blood (Buffy coat), BAL, CSF, urine, amniotic fluid and in immunohistochemistry on lung, liver, kidney, colon, brain, nerve... SEE ALSO CONTROL SLIDES.

This monoclonal antibody recognizes an Early Antigen which can be detected within 12 hours after cell infection. No cross-reactivity with HSV-1, HSV-2, VZV, EBV and ADV. The antibody shows a nuclear staining. This antibody is not suitable for use in Antigenemia. SEE ALSO CONTROL SLIDES.

This murine monoclonal antibody recognizes a Late Antigen which is at a sufficient level for detection 72 hours after cell infection. The technique using this antibody allows the follow-up of isolates sensitivity to antiviral drugs by measuring the decreasing number of infected cells producing this late antigen in presence of increasing concentrations of drug. IC50 and IC90 are then determined. No cross-reactivity with other Herpesviridae nor ADV.